How to use a microscope
  • 1. What is the primary function of a microscope?
A) To dissect specimens
B) To illuminate a room
C) To magnify small objects
D) To measure distances
  • 2. Which part of the microscope focuses light onto the specimen?
A) Objective lens
B) Stage
C) Condenser
D) Eyepiece
  • 3. What is the name of the lens you look through?
A) Diaphragm
B) Objective lens
C) Eyepiece
D) Condenser lens
  • 4. Which adjustment knob is used for coarse focusing?
A) Coarse adjustment knob
B) Fine adjustment knob
C) Stage adjustment knob
D) Condenser adjustment knob
  • 5. What is the purpose of the stage clips?
A) To adjust the light intensity
B) To focus the image
C) To hold the slide in place
D) To move the stage
  • 6. Which part controls the amount of light passing through the specimen?
A) Coarse focus knob
B) Diaphragm
C) Objective lens
D) Eyepiece
  • 7. What does 'parfocal' mean in relation to microscope objective lenses?
A) The image is always perfectly clear
B) The image stays in focus when changing objectives
C) The magnification is consistent
D) The lenses are all made of glass
  • 8. How should you carry a microscope?
A) By the stage
B) With one hand on the arm and the other under the base
C) By the eyepiece
D) By the objective lenses
  • 9. What should you use to clean the microscope lenses?
A) Paper towel
B) Facial tissue
C) Lens paper
D) Cloth
  • 10. When using the highest power objective, what adjustment knob should you use?
A) Stage adjustment knob
B) Condenser adjustment knob
C) Fine adjustment knob
D) Coarse adjustment knob
  • 11. What is the total magnification when using a 10x eyepiece and a 40x objective?
A) 400x
B) 1040x
C) 50x
D) 4x
  • 12. What is the first step in preparing a wet mount slide?
A) Place a drop of liquid on the slide
B) Add stain to the specimen
C) Clean the slide
D) Place the coverslip on the slide
  • 13. What is the purpose of a coverslip?
A) To add contrast to the specimen
B) To hold the slide in place
C) To magnify the specimen
D) To flatten the specimen and protect the objective lens
  • 14. Which type of microscope is used to observe living cells in their natural state?
A) Scanning electron microscope
B) Transmission electron microscope
C) Compound microscope
D) Phase contrast microscope
  • 15. What is the purpose of oil immersion?
A) To clean the objective lens
B) To increase resolution at high magnification
C) To reduce the amount of light needed
D) To stain the specimen
  • 16. Before storing the microscope, what objective should be in place?
A) Lowest power objective
B) Any objective
C) Highest power objective
D) Oil immersion objective
  • 17. What does resolution refer to?
A) The depth of focus
B) The brightness of the image
C) The magnification power of the microscope
D) The ability to distinguish between two closely spaced objects
  • 18. What should you do if you can't see anything when looking through the microscope?
A) Increase the magnification
B) Clean the eyepiece
C) Add more stain
D) Check the light source and objective lens position
  • 19. What is the function of the arm of the microscope?
A) Controls the light source
B) Holds the objective lenses
C) Supports the body tube and is used for carrying
D) Supports the stage
  • 20. The base of the microscope...
A) Provides a stable platform for the microscope
B) Adjusts the focus
C) Holds the objective lenses
D) Contains the light source
  • 21. What is the field of view?
A) The visible area seen through the eyepiece
B) The objective lens in use
C) The brightness of the light source
D) The magnification power
  • 22. What is the working distance?
A) The length of the microscope arm
B) The distance between the objective lens and the specimen
C) The distance between the eyepieces
D) The field of view diameter
  • 23. Why is it important to center the specimen before increasing magnification?
A) To reduce the amount of light needed
B) To increase the resolution
C) To prevent the specimen from moving out of the field of view
D) To improve the contrast
  • 24. What is the refractive index?
A) The amount of light produced by the light source
B) A measure of how much light bends when it passes from one medium to another
C) The strength of the objective lens
D) The size of the specimen being observed
  • 25. What is the role of immersion oil in microscopy?
A) To clean the objective lens
B) To enhance the color of the specimen
C) To minimize light refraction and improve resolution
D) To act as a mounting medium for the specimen
  • 26. What is the numerical aperture (NA) of a microscope objective?
A) The distance between the lens and the specimen
B) The magnification power of the lens
C) A measure of the lens' ability to gather light and resolve fine specimen detail
D) The field of view diameter
  • 27. What is Köhler illumination?
A) A type of stain used in microscopy
B) A technique for optimizing the light path in a microscope to produce a high-quality image
C) A type of microscope objective
D) A method for cleaning microscope lenses
  • 28. What type of illumination is used in a darkfield microscope?
A) Light is scattered by the specimen and enters the objective lens
B) Direct light passes through the specimen
C) Polarized light is used to illuminate the specimen
D) Fluorescent light is used to illuminate the specimen
  • 29. What is fluorescence microscopy used for?
A) To perform microsurgery
B) To observe the surface topography of a specimen
C) To visualize specific structures or molecules within a cell or tissue
D) To measure the electrical properties of a cell
  • 30. What is a microtome used for?
A) To cut thin sections of tissue for microscopy
B) To measure the size of cells
C) To sterilize microscope slides
D) To mix reagents for staining
Created with That Quiz — where test making and test taking are made easy for math and other subject areas.