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Supercritical Fluid Chromatography
  • 1. Which of the following is commonly used as the mobile phase in SFC?
A) Carbon dioxide
B) Water
C) Acetone
D) Methanol
  • 2. What state is the mobile phase in SFC?
A) Supercritical
B) Solid
C) Liquid
D) Gas
  • 3. What is the mechanism of separation in SFC based on?
A) Differences in solubility in the mobile phase
B) Differences in boiling point
C) Differences in conductivity
D) Differences in molecular weight
  • 4. What advantage does SFC offer over traditional HPLC?
A) Faster separation
B) Limited application range
C) Higher resolution
D) Lower equipment cost
  • 5. At supercritical conditions, the density of carbon dioxide is similar to that of which phase?
A) Solid
B) Gas
C) Liquid
D) Aqueous
  • 6. What is the purpose of using a back pressure regulator in SFC?
A) Avoid solvent contamination
B) Increase column temperature
C) Maintain supercritical conditions
D) Enhance detector sensitivity
  • 7. What role does co-solvent play in SFC separations?
A) Improves stationary phase selectivity
B) Stabilizes column efficiency
C) Enhances analyte solubility
D) Reduces detector interference
  • 8. Which term describes the region where the mobile phase in SFC exhibits properties of both a liquid and a gas?
A) Supercritical zone
B) Near-critical region
C) Transition zone
D) Critical point
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