Supercritical Fluid Chromatography
  • 1. Which of the following is commonly used as the mobile phase in SFC?
A) Carbon dioxide
B) Methanol
C) Acetone
D) Water
  • 2. What state is the mobile phase in SFC?
A) Supercritical
B) Solid
C) Gas
D) Liquid
  • 3. What is the mechanism of separation in SFC based on?
A) Differences in solubility in the mobile phase
B) Differences in boiling point
C) Differences in conductivity
D) Differences in molecular weight
  • 4. What is the purpose of using a back pressure regulator in SFC?
A) Increase column temperature
B) Avoid solvent contamination
C) Enhance detector sensitivity
D) Maintain supercritical conditions
  • 5. What advantage does SFC offer over traditional HPLC?
A) Higher resolution
B) Lower equipment cost
C) Faster separation
D) Limited application range
  • 6. What role does co-solvent play in SFC separations?
A) Enhances analyte solubility
B) Reduces detector interference
C) Improves stationary phase selectivity
D) Stabilizes column efficiency
  • 7. Which term describes the region where the mobile phase in SFC exhibits properties of both a liquid and a gas?
A) Transition zone
B) Critical point
C) Near-critical region
D) Supercritical zone
  • 8. At supercritical conditions, the density of carbon dioxide is similar to that of which phase?
A) Gas
B) Solid
C) Aqueous
D) Liquid
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