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Supercritical Fluid Chromatography
  • 1. Which of the following is commonly used as the mobile phase in SFC?
A) Carbon dioxide
B) Water
C) Methanol
D) Acetone
  • 2. What state is the mobile phase in SFC?
A) Liquid
B) Solid
C) Supercritical
D) Gas
  • 3. What is the mechanism of separation in SFC based on?
A) Differences in solubility in the mobile phase
B) Differences in molecular weight
C) Differences in boiling point
D) Differences in conductivity
  • 4. What advantage does SFC offer over traditional HPLC?
A) Lower equipment cost
B) Higher resolution
C) Limited application range
D) Faster separation
  • 5. At supercritical conditions, the density of carbon dioxide is similar to that of which phase?
A) Liquid
B) Solid
C) Gas
D) Aqueous
  • 6. What is the purpose of using a back pressure regulator in SFC?
A) Increase column temperature
B) Enhance detector sensitivity
C) Maintain supercritical conditions
D) Avoid solvent contamination
  • 7. What role does co-solvent play in SFC separations?
A) Enhances analyte solubility
B) Reduces detector interference
C) Improves stationary phase selectivity
D) Stabilizes column efficiency
  • 8. Which term describes the region where the mobile phase in SFC exhibits properties of both a liquid and a gas?
A) Critical point
B) Supercritical zone
C) Near-critical region
D) Transition zone
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