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Supercritical Fluid Chromatography
Contributed by: Porter
  • 1. Which of the following is commonly used as the mobile phase in SFC?
A) Carbon dioxide
B) Methanol
C) Water
D) Acetone
  • 2. What state is the mobile phase in SFC?
A) Gas
B) Solid
C) Liquid
D) Supercritical
  • 3. What is the mechanism of separation in SFC based on?
A) Differences in solubility in the mobile phase
B) Differences in conductivity
C) Differences in boiling point
D) Differences in molecular weight
  • 4. What advantage does SFC offer over traditional HPLC?
A) Faster separation
B) Lower equipment cost
C) Higher resolution
D) Limited application range
  • 5. At supercritical conditions, the density of carbon dioxide is similar to that of which phase?
A) Liquid
B) Aqueous
C) Gas
D) Solid
  • 6. What is the purpose of using a back pressure regulator in SFC?
A) Increase column temperature
B) Enhance detector sensitivity
C) Avoid solvent contamination
D) Maintain supercritical conditions
  • 7. What role does co-solvent play in SFC separations?
A) Enhances analyte solubility
B) Improves stationary phase selectivity
C) Reduces detector interference
D) Stabilizes column efficiency
  • 8. Which term describes the region where the mobile phase in SFC exhibits properties of both a liquid and a gas?
A) Supercritical zone
B) Critical point
C) Near-critical region
D) Transition zone
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