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Supercritical Fluid Chromatography
Contributed by: Porter
  • 1. Which of the following is commonly used as the mobile phase in SFC?
A) Water
B) Acetone
C) Methanol
D) Carbon dioxide
  • 2. What state is the mobile phase in SFC?
A) Gas
B) Supercritical
C) Solid
D) Liquid
  • 3. What is the mechanism of separation in SFC based on?
A) Differences in conductivity
B) Differences in molecular weight
C) Differences in boiling point
D) Differences in solubility in the mobile phase
  • 4. What advantage does SFC offer over traditional HPLC?
A) Higher resolution
B) Lower equipment cost
C) Faster separation
D) Limited application range
  • 5. At supercritical conditions, the density of carbon dioxide is similar to that of which phase?
A) Gas
B) Liquid
C) Aqueous
D) Solid
  • 6. What is the purpose of using a back pressure regulator in SFC?
A) Enhance detector sensitivity
B) Maintain supercritical conditions
C) Increase column temperature
D) Avoid solvent contamination
  • 7. What role does co-solvent play in SFC separations?
A) Reduces detector interference
B) Improves stationary phase selectivity
C) Stabilizes column efficiency
D) Enhances analyte solubility
  • 8. Which term describes the region where the mobile phase in SFC exhibits properties of both a liquid and a gas?
A) Near-critical region
B) Supercritical zone
C) Transition zone
D) Critical point
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